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Team:DTU-Denmark/Notebook/6 September 2013
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | Template: HAO extraction fragment -isolated from 'Nitrosomonas europea'- (purified from PCR run on 04.09) | ||
| + | Primers: 18a & 18b | ||
| + | Two versions: one with 5% DMSO and one with 3% DMSO | ||
| + | PCR reaction master mix (x5) | ||
| + | {| class="wikitable" style="text-align: right" | ||
| + | ! compound !! volume (uL) | ||
| + | |- | ||
| + | | dNTPs|| 1 | ||
| + | |- | ||
| + | | HF || 10 | ||
| + | |- | ||
| + | | polymerase x7 || 0.5 | ||
| + | |- | ||
| + | | FW || 3 | ||
| + | |- | ||
| + | | RV || 3 | ||
| + | |- | ||
| + | | template || 1 | ||
| + | |- | ||
| + | | MQ || 29 | ||
| + | |- | ||
| + | | DMSO || 1.5 | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | *Add 49 ul of the master mix, and | ||
| + | *Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%) | ||
==Results== | ==Results== | ||
Revision as of 08:40, 6 September 2013
6 September 2013
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Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Template: HAO extraction fragment -isolated from 'Nitrosomonas europea'- (purified from PCR run on 04.09) Primers: 18a & 18b Two versions: one with 5% DMSO and one with 3% DMSO
PCR reaction master mix (x5)
| compound | volume (uL) |
|---|---|
| dNTPs | 1 |
| HF | 10 |
| polymerase x7 | 0.5 |
| FW | 3 |
| RV | 3 |
| template | 1 |
| MQ | 29 |
| DMSO | 1.5 |
- Add 49 ul of the master mix, and
- Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%)
Results
Conclusion
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