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Team:DTU-Denmark/Notebook/6 September 2013
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Two versions: one with 5% DMSO and one with 3% DMSO | Two versions: one with 5% DMSO and one with 3% DMSO | ||
| - | PCR reaction master mix | + | (x5) |
| + | [[Team:DTU-Denmark/Methods/PCR-mix|PCR reaction master mix]] | ||
| + | [[Team:DTU-Denmark/Methods/Determining_concentration_of_nitrogen_compounds/Experiment_2|Experiment 2]] | ||
{| class="wikitable" style="text-align: right" | {| class="wikitable" style="text-align: right" | ||
! compound !! volume (uL) | ! compound !! volume (uL) | ||
Revision as of 08:46, 6 September 2013
6 September 2013
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Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Template: HAO extraction fragment -isolated from 'Nitrosomonas europea'- (purified from PCR run on 04.09) Primers: 18a & 18b Two versions: one with 5% DMSO and one with 3% DMSO
(x5)
PCR reaction master mix Experiment 2
| compound | volume (uL) |
|---|---|
| dNTPs | 1 |
| HF | 10 |
| polymerase x7 | 0.5 |
| FW | 3 |
| RV | 3 |
| template | 1 |
| MQ | 29 |
| DMSO | 1.5 |
- Add 49 ul of the master mix, and
- Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%)
Results
Conclusion
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