Team:DTU-Denmark/Notebook/6 September 2013

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==Main purpose==
==Main purpose==
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*Add USER endings to HAO gene extracted from 'Nitrosomonas europea'
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*Add USER endings to HAO gene extracted from ''Nitrosomonas europea''
==Who was in the lab==
==Who was in the lab==

Revision as of 08:52, 6 September 2013

6 September 2013

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Contents

Lab 208


Main purpose


  • Add USER endings to HAO gene extracted from Nitrosomonas europea

Who was in the lab



Procedure


Template: HAO extraction fragment -isolated from Nitrosomonas europea- (purified from PCR run on 04.09)

Primers: 18a & 18b

Two versions: one with 5% DMSO and one with 3% DMSO

PCR reaction master mix

compound volume (uL)
dNTPs 1
HF 10
polymerase x7 0.5
FW 3
RV 3
template 1
MQ 29
DMSO 1.5
  • Add 49 ul of the master mix, and
  • Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%)

Results


Conclusion



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