Team:DTU-Denmark/Notebook/6 September 2013

From 2013.igem.org

Revision as of 08:51, 6 September 2013 by Biotacg (Talk | contribs)

6 September 2013

Navigate to the Previous or the Next Entry

Contents

Lab 208


Main purpose


  • Add USER endings to HAO gene extracted from 'Nitrosomonas europea'

Who was in the lab



Procedure


Template: HAO extraction fragment -isolated from 'Nitrosomonas europea'- (purified from PCR run on 04.09)

Primers: 18a & 18b

Two versions: one with 5% DMSO and one with 3% DMSO

PCR reaction master mix

compound volume (uL)
dNTPs 1
HF 10
polymerase x7 0.5
FW 3
RV 3
template 1
MQ 29
DMSO 1.5
  • Add 49 ul of the master mix, and
  • Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%)

Results


Conclusion



Navigate to the Previous or the Next Entry