Team:DTU-Denmark/Notebook/7 June 2013

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{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}}
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=lab 208=
=lab 208=
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<hr/>
== Main purposes today ==
== Main purposes today ==
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<hr/>
*helloworld-project
*helloworld-project
*prepare solutions
*prepare solutions
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
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*plate ''E.coli''
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==who were in the lab==
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==Who was in the lab==
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<hr/>
Kristian
Kristian
==Procedure==
==Procedure==
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<hr/>
#1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
#1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
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#50% glycerol (500ml)
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#50% glycerol (500 ml)
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#LB medium (liquid) (3 L);
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->20 g of LB in every 1 L of distilled water. Autoclaved medium
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->in 1 of these L, 14 g of agar were added for making LB solid medium
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===plating E.coli===
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*Colonies of Top 10 ''E.coli'' were streaked on two plates
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*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
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Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry
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{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 20:59, 16 September 2013

07 June 2013

Contents

lab 208


Main purposes today


  • helloworld-project
  • prepare solutions
  • autoclaving
  • plate E.coli

Who was in the lab


Kristian

Procedure


  1. 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
  2. 50% glycerol (500 ml)
  3. LB medium (liquid) (3 L);

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

plating E.coli

  • Colonies of Top 10 E.coli were streaked on two plates
  • Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

Navigate to the Previous or the Next Entry