Team:DTU-Denmark/Notebook/7 June 2013

From 2013.igem.org

(Difference between revisions)
(Procedure)
 
(6 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}}
{{:Team:DTU-Denmark/Templates/StartPage|07 June 2013}}
-
__TOC__
 
=lab 208=
=lab 208=
Line 9: Line 8:
*prepare solutions
*prepare solutions
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
*[[Team:DTU-Denmark/Methods/Autoclaving|autoclaving]]
-
*plate E.coli
+
*plate ''E.coli''
==Who was in the lab==
==Who was in the lab==
Line 24: Line 23:
->in 1 of these L, 14 g of agar were added for making LB solid medium
->in 1 of these L, 14 g of agar were added for making LB solid medium
-
=jfla=
+
===plating E.coli===
 +
*Colonies of Top 10 ''E.coli'' were streaked on two plates
 +
*Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)
Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry
Navigate to the [[Team:DTU-Denmark/Notebook/5_June_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/9_June_2013|Next]] Entry
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 20:59, 16 September 2013

07 June 2013

Contents

lab 208


Main purposes today


  • helloworld-project
  • prepare solutions
  • autoclaving
  • plate E.coli

Who was in the lab


Kristian

Procedure


  1. 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
  2. 50% glycerol (500 ml)
  3. LB medium (liquid) (3 L);

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

plating E.coli

  • Colonies of Top 10 E.coli were streaked on two plates
  • Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

Navigate to the Previous or the Next Entry