Team:DTU-Denmark/Notebook/7 June 2013

From 2013.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)

Latest revision as of 20:59, 16 September 2013

07 June 2013

Contents

lab 208


Main purposes today


  • helloworld-project
  • prepare solutions
  • autoclaving
  • plate E.coli

Who was in the lab


Kristian

Procedure


  1. 1M CaCl_2 (200ml) weighed off 22.206 g CaCl_2
  2. 50% glycerol (500 ml)
  3. LB medium (liquid) (3 L);

->20 g of LB in every 1 L of distilled water. Autoclaved medium

->in 1 of these L, 14 g of agar were added for making LB solid medium

plating E.coli

  • Colonies of Top 10 E.coli were streaked on two plates
  • Colonies of Top 10 + pZE21 em GFP were streaked on kanamycin-Agar -> 10 uL kan on each plate (LB + 30 ug/mL kan)

Navigate to the Previous or the Next Entry