Team:DTU-Denmark/Notebook/9 July 2013

From 2013.igem.org

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==Procedure==
==Procedure==
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===Run gel:===  
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===Run gel===
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* 1ul of dye
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* 5ul of sample
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* 5ul Kb ladder
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===Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)===
===Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)===

Revision as of 15:35, 9 July 2013

Contents

208

Main purpose

Run gel with 7 Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.

Purification of Nir operon and Nanodrop measurement.

Who was in the lab

Ariadni,,Henrike,Julia

Procedure

Run gel

Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)

PCR

Results

Nanodrop measurement:

  • ng/ul (Nir)
  • ng/ul (Nir)
  • ng/ul (Nir)