Team:DTU-Denmark/Notebook/9 July 2013

From 2013.igem.org

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(Main purpose)
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=208=
=208=
==Main purpose==
==Main purpose==
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Run gel with 7 PCR samples of Nir operon from "Pseudomonas aeruginosa" and purified AMO, HAO and CYC from 08.07.2013.
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Run gel with 7 PCR samples of Nir operon from ''Pseudomonas aeruginosa'' and purified AMO, HAO and CYC from 08.07.2013.
Purification of Nir operon and Nanodrop measurement.
Purification of Nir operon and Nanodrop measurement.
 +
 +
Set up new PCR reaction to extract Nir from ''Pseudomonas aeruginosa''.
==Who was in the lab==
==Who was in the lab==

Revision as of 17:14, 9 July 2013

Contents

208

Main purpose

Run gel with 7 PCR samples of Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.

Purification of Nir operon and Nanodrop measurement.

Set up new PCR reaction to extract Nir from Pseudomonas aeruginosa.

Who was in the lab

Ariadni,Henrike,Julia

Procedure

Run gel

Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)

PCR

Results

Nanodrop measurement:

  • ng/ul (Nir)
  • ng/ul (Nir)
  • ng/ul (Nir)