Team:DTU-Denmark/Notebook/9 September 2013

From 2013.igem.org

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(Procedure)
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==Results==
==Results==
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===Transfomation===
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Transformation on last friday gave the following results:
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*HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
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*negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
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*HAO in pZA21::ara tight - a lot of colonies, some very big
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*negative HAO in pZA21::ara tight - a lot of small colonies
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*Nir in pZA21::ara tight - 8 colonies
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*negative Nir in pZA21::ara tight - no colonies
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Revision as of 15:30, 9 September 2013

9 September

Contents

lab 208


Main purpose


Who was in the lab


Julia, Kristian, Henrike

Procedure


Colony PCR to verify inserts HAO and Nir in pZA21::ara and pZA21::ara tight

Results


Transfomation

Transformation on last friday gave the following results:

  • HAO in pZA21::ara (from Biobrick K808000) - 6 colonies (3 orange and small, 3 normal color and very big)
  • negative HAO in pZA21::ara (from Biobrick K808000) - no colonies
  • HAO in pZA21::ara tight - a lot of colonies, some very big
  • negative HAO in pZA21::ara tight - a lot of small colonies
  • Nir in pZA21::ara tight - 8 colonies
  • negative Nir in pZA21::ara tight - no colonies