Team:DTU-Denmark/Project

From 2013.igem.org

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(Project Details)
(Overall project)
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== '''Overall project''' ==
 
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
 
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==Abstract==
Our project simultaneously cleans and reclaims energy from waste water streams by using two E. coli mutants to turn ammonia into nitrous oxide.
Our project simultaneously cleans and reclaims energy from waste water streams by using two E. coli mutants to turn ammonia into nitrous oxide.
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The final product is nitrous oxide, which can be reclaimed and used as a fuel source.   
The final product is nitrous oxide, which can be reclaimed and used as a fuel source.   
In effect, we are turning pollution into energy.
In effect, we are turning pollution into energy.
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==Details==
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[[File:DTU_Pathway_overview.png]]
== Bioinformatics ==
== Bioinformatics ==

Revision as of 16:53, 9 July 2013


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Contents

Abstract

Our project simultaneously cleans and reclaims energy from waste water streams by using two E. coli mutants to turn ammonia into nitrous oxide.

DTU Project sketch v2.png

Industrial processes have greatly increased the amount of fixed nitrogen, leading to an excess of ammonia in the environment. Ammonia is a common pollutant in agricultural runoff, and excess in the environment leads to increased algae growth (eutrophication) that potentially starves other water-borne organisms for oxygen. Our project reverses nitrogen fixation, and is a means to clean up polluted streams and wastewater. The final product is nitrous oxide, which can be reclaimed and used as a fuel source. In effect, we are turning pollution into energy.


Details

DTU Pathway overview.png

Bioinformatics

Aligning signal peptides

 >HAO
 M-RIGE---------WMRGLLL-CA--GLMMCGVVH--A
 >nirN
 ----------------MRLIDL-AL--GLLLGALAQ--A
 >nirS
 M-PFGK---------PLAGTLLASL--TLLGLATAH--A
 >nirM
 M----K---------PYALLSLLAT--GTLLAQGAW--A
 >cc554/cycA
 M----K---------IMIACGLVAA--ALFTLTSGQSLA
 >ccm552/cycX
 MTRLQK---------GSIGTLLTGALLGIVLVAVVF--G
 >sec
 M-NIKK---------FAKQATVLTFTTALLAGGATQAFA
 >nirC
 MNAPPDFRRAASHALWLALALTFA--------CPLPGLA
 >tat_E_coli
 MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATA

Results