Team:DTU-Denmark/Timeline

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Timeline


Contents

Project Timeline

January

February

  • 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in E. coli

March

  • 2013-03-11 Biobrick workshop hosted for KU and SDU
  • 2013-03-13 Project kickoff meeting with supervisors

April

May

June

  • 2013-06-04 Team competency mapping
  • 2013-06-09 Work on wiki started
  • 2013-06-29 Team BBQ

July

Week 27

  • Hello World project complete and successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm.
  • Toxicity Experiment: experiment complete, initial data analysis complete. See results at: here

Week 28

  • Began growing Nitrosomonas culture and made special media for it
  • Designed, ordered and received primers to extract genes from Nitrosomonas and Pseudomonas
  • Began work on cloning genes out of Nitrosomonas europaea and Pseudomonas aeruginosa. All isolations successful except Nir.

Week 29

  • Performed first nitrate -> nitrite experiment

Week 30

  • Only have colonies for the cytochromes -- other genes do not give cultures
  • Attempting to extract Nir gene in two pieces, since this has not been successful so far
  • Began work on arabinose inducible system

Week 31

  • Began work on Synthetic Promoter Library
  • BBQ with KU this Saturday

August

Week 32

  • The week no PCR worked -- ran 96 PCRs with varying conditions to debug
  • Began work on modelling
  • Began work on presentation

Week 33

  • Began daily meetings to coordinate
  • Arabinose inducible promoter works as of 2013-08-11
  • One Nir fragment has been amplified with USER primers (changed DMSO, Mg, temperature, etc), one to go
  • E coli experiment will be run again this week

Week 34

  • Decided on final human practices event

Week 35

  • Attended Scandinavian meetup in Odense hosted by SDU team
  • Biobricks in progress
  • Biolector for SPL in progress
  • sequencing for SPL in progress
  • first draft of presentation
  • first draft of poster
  • measure Nir, AMO, HAO transformants
  • modelling of kinetics and design of a reactor: Vanessa
  • Title and abstract finished; Title: Requiem for a Stream
  • Reviewed presentation with Julie, who gave us very good advice on pitching our project
  • measure AMO, HAO transformants complete

September

Week 36

  • Have biobricks for AMO and cytochromes (are going to sequence these), the others are coming.
  • Running last biolector experiment today
  • sequencing for SPL is in progress
  • Kristian is working on a page in the parts registry for the parts registry
  • first draft of presentation completed
  • first draft of poster complete
  • modelling of kinetics and design of a reactor: using Continuous Stirred Tank Reactor
  • Began reactor design -- ask Mikael Rordham in 223 for 2 1L chemostats

Week 37

  • biobricks -- have 2 that are fine: AMO and cytochromes
  • redo colony pcr with sequencing primers to verify
  • Nir experiment on friday
  • Planning for bioreactor experiment
  • Human Practices: Attended BioPeople event "The Basics and the Best" on IP and patent law

Week 38

  • Filmed "Bricks of Knowledge" video on USER cloning for KU team
  • Biobricks mailed
    • tat signal peptide
    • amo
    • cytochromes
  • arabinose promoter biobrick not made -- Kristian and Henrike will make this in the next few days
  • Bioreactor experiment started this morning, will finish at 10pm tonight.

Week 39

  • Hackathon intensive to complete wiki 28th of Sept
  • Nir experiment -- Kasia and Ariadne will do this on Thursday
  • Video in progress
  • Reviewed poster v2

October

Week 40

  • Wiki complete!