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Project Timeline



  • 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in E. coli.


  • 2013-03-11 Biobrick workshop hosted for KU and SDU.
  • 2013-03-13 Project kickoff meeting with supervisors.




  • 2013-06-04 Team competency mapping.
  • 2013-06-09 Work on wiki started.
  • 2013-06-29 Team BBQ.


Week 27

  • Hello World project complete and successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm.
  • Conducted the toxicity experiment and completed data analysis: average ammonia concentrations in wastewater are not toxic to E. coli.

Week 28

  • Designed, ordered and received primers to extract genes from Nitrosomonas and Pseudomonas.
  • Began work on cloning genes out of Nitrosomonas europaea and Pseudomonas aeruginosa. All isolations successful so far except for Nir.
  • Held the first hackaton to work on the wiki.

Week 29

Week 30

  • Ordered new primers to extract Nir gene in two pieces.
  • Despite successful PCR and transformation of AMO, HAO and cytochromes, we only have viable colonies for the cytochromes. Expect that this is due to the fact that we are expressing periplasmic and membrane proteins, which is causing excessive stress on the cells.
  • Began work on pBAD synthetic promoter library so that we will be able to induce a low level of expression of our periplasmic genes.

Week 31

  • The week no PCR worked -- ran 96 PCRs with varying conditions to debug what is not working.
  • Saturday BBQ with KU.


Week 32

Week 33

Week 34

Week 35

  • Attended Scandinavian meetup in Odense hosted by SDU team. Thank you SDU for a great weekend!
  • Conducted another set of biolector experiments to characterize more colonies from the pBAD synthetic promoter library.
  • Sent pBAD SPL samples for sequencing.
  • Conducted experiment to characterize AMO and HAO transformants. Found that the AMO transformant is using ammonia faster than a nontransformed E. coli control!
  • Decided on project title: Requiem for a Stream.
  • Wrote the project abstract.
  • Reviewed presentation with Julie, who gave us very good advice on pitching our project.
  • Completed safety form.


Week 36

Week 37

Week 38

Week 39


Week 40

  • Safety forms were approved on September 29, 2013 by the iGEM Safety Committee.
  • Completed wiki.