"
Team:DTU-Denmark/pBAD SPL
From 2013.igem.org
(Difference between revisions)
(→pBAD synthetic promoter library) |
(→pBAD synthetic promoter library) |
||
| Line 3: | Line 3: | ||
== pBAD synthetic promoter library == | == pBAD synthetic promoter library == | ||
| - | As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL link]. | + | As a tool for expressing lethal proteins in ''E.coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 [https://2010.igem.org/Team:DTU-Denmark/SPL (link)]. |
| + | == Method == | ||
| + | === USER cloning for SPL === | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | == Results == | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | == Example of use == | ||
[[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm.png|thumbnail|upright=4|left|alt=Alt text|Caption text]] | ||
[[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | [[File:GFP in perimplasm RFP in cytoplasm close up.png|thumbnail|upright=2|left|alt=Alt text|Caption text]] | ||
[[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]] | [[File:Graf.PNG|thumbnail|upright=2|left|alt=Alt text|Caption text]] | ||
| + | |||
| + | See also [[HelloWorld]]. | ||
| + | |||
Revision as of 19:00, 28 September 2013
pBAD SPL
Contents |
pBAD synthetic promoter library
As a tool for expressing lethal proteins in E.coli we made a synthetic promoter library (SPL, RFC 63) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 (link).
Method
USER cloning for SPL
Results
Example of use
See also HelloWorld.
