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Team:DTU-Denmark/pBAD SPL
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== pBAD synthetic promoter library == | == pBAD synthetic promoter library == | ||
| - | As a tool for expressing lethal proteins in ''E. coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the | + | As a tool for expressing lethal proteins in ''E. coli'' we made a synthetic promoter library (SPL, [http://dspace.mit.edu/handle/1721.1/60080 RFC 63]) with the pBAD arabinose inducible promoter. The concept was taken from the [https://2010.igem.org/Team:DTU-Denmark/SPL DTU iGEM team from 2010]. |
== Method == | == Method == | ||
Revision as of 20:25, 30 September 2013
pBAD SPL
Contents |
pBAD synthetic promoter library
As a tool for expressing lethal proteins in E. coli we made a synthetic promoter library (SPL, RFC 63) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010.
Method
USER cloning for SPL
Results
Example of use
See also "Hello World project".
