Team:DTU-Denmark/pBAD SPL

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pBAD SPL

Contents

1 pBAD synthetic promoter library
2 Method
- 2.1 USER cloning for SPL
3 Results
4 Example of use

pBAD synthetic promoter library

As a tool for expressing lethal proteins in E. coli we made a synthetic promoter library (SPL, RFC 63) with the pBAD arabinose inducible promoter. The concept was taken from the DTU iGEM team from 2010 (link).

Method

USER cloning for SPL

Results

Example of use

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See also "Hello World project".

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