Team:Dundee

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        <h2 style="margin-top:-10px;"> Targeting a Deadly Toxin</h2>
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        Explosions in the population of cyanobacteria can produce toxic algal blooms. Microcystin-LR the most potent and common algal bloom toxin, binds Protein Phosphatase 1. The average cyanobacteria infested lake in America contains over 1000 times the Microcystin safe drinking water limit set by the World Health Organisation. <br><br>
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        Concerned by a harmful algal bloom in the local community, we used synthetic biology to target the toxin. We exploited the mechanism of Microcystin's toxicity to develop our Mop; by expressing Protein Phosphatase 1 we can mop up Microcystin. The interaction was also the basis for developing a biological Detector. To deploy our Detector and to consider the root causes of algal blooms we created the electronic Moptopus. It sits on a lake and monitors conditions relevant to cyanobacterial growth to help predict future blooms.
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          <li class="active"><a href="/Team:Dundee">Home</a></li>
 
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                          <p><b style="font-size:16px">Mop</b><br><br> Mopping up a toxin (microcystin) by engineering a bacterium to produce the human PP1 protein, the protein to which microcystin binds.</p>
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            <h5 class="subheader">Algal blooms are an ever-growing problem in freshwater systems. The main concern is the level of toxin called microcystin, which is released by cyanobacteria when they die. Currently, the method of detection takes a day to produce results, so our aim as a team is to develop a 60 minute microcystin detection system, as well as a method to combat the rising levels of the toxin.</h5>
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                          <p><b style="font-size:16px;">Detector</b><br><br> Detecting microcystin by manipulation of the <i>E. coli</i> osmoregulator EnvZ.</p>
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                          <p><b style="font-size:16px;">Moptopus</b><br><br>  An electronic sensing device that provides a platform for toxin detection. It relates a range of environmental conditions to algal bloom formation and toxicity.</p>
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              <p><b>Description</b> - Aliquam erat volutpat. Mauris ultricies et iusto odio dignissimos ducimus posuere vehicula.</p>
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                          <p><b style="font-size:16px;">Human Practices</b><br><br>This project has been carried out in collaboration with the community. By informing, listening and responding to their input, our project is based around community defined need, and is not merely a technical exercise. </p>
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Latest revision as of 23:35, 28 October 2013

iGEM Dundee 2013 · ToxiMop

Dundee 2013

Targeting a Deadly Toxin

Explosions in the population of cyanobacteria can produce toxic algal blooms. Microcystin-LR the most potent and common algal bloom toxin, binds Protein Phosphatase 1. The average cyanobacteria infested lake in America contains over 1000 times the Microcystin safe drinking water limit set by the World Health Organisation.

Concerned by a harmful algal bloom in the local community, we used synthetic biology to target the toxin. We exploited the mechanism of Microcystin's toxicity to develop our Mop; by expressing Protein Phosphatase 1 we can mop up Microcystin. The interaction was also the basis for developing a biological Detector. To deploy our Detector and to consider the root causes of algal blooms we created the electronic Moptopus. It sits on a lake and monitors conditions relevant to cyanobacterial growth to help predict future blooms.



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