Team:EPF Lausanne/Calendar/12 September 2013

From 2013.igem.org

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{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
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<font size = "4"> Cell Surface Display </font> <BR>
-
Sensing <BR>
+
''Inverted Microscopy'' <BR>
 +
- All our constructs (ISA,ISD,ISI and competent cells as negative control) were prepared immonustaining assays and biotin staining assays.
 +
<br>- Visualization under the microscope with a FITC filter.
 +
<br>- BBa_K523013 construct was also better characterized with an anti-GFP antibody under the microscope (red filter).
 +
<br>- Inoculation overnight of all our constructs for functional assay involving nanoparticles.
 +
<br><br>
-
'''Test PCR for the constitutive promoter and the GelE insert''' <BR>
+
<font size = "4"> Sensing-Effector </font> <BR>
-
I did a PCR of the MiniPrep and it showed that the plasmid did indeed contain the inserts we wanted.
+
-
'''Sequencing''' <BR>
+
''Test PCR for the constitutive promoter and the GelE insert'' <BR>
-
I sent all the constructs for sequencing.
+
-I did a PCR of the MiniPrep and it showed that the plasmid did indeed contain the inserts we wanted.
 +
''Sequencing'' <BR>
 +
-I sent all the constructs for sequencing.
 +
<BR><BR>
-
'''Nanoparticles'''
+
<font size = "4"> Nanoparticles</font> <BR>
-
<BR>- Washing of the two new nanoparticles batches
+
 
 +
''Washing''<BR>
 +
-We washed the two new nanoparticles batches.
<BR>- New nanoparticles DLS measurements
<BR>- New nanoparticles DLS measurements
<BR>- Microscopy (visualisation of the new loaded NPs)
<BR>- Microscopy (visualisation of the new loaded NPs)

Latest revision as of 22:21, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Inverted Microscopy
- All our constructs (ISA,ISD,ISI and competent cells as negative control) were prepared immonustaining assays and biotin staining assays.
- Visualization under the microscope with a FITC filter.
- BBa_K523013 construct was also better characterized with an anti-GFP antibody under the microscope (red filter).
- Inoculation overnight of all our constructs for functional assay involving nanoparticles.

Sensing-Effector

Test PCR for the constitutive promoter and the GelE insert
-I did a PCR of the MiniPrep and it showed that the plasmid did indeed contain the inserts we wanted.

Sequencing
-I sent all the constructs for sequencing.

Nanoparticles

Washing
-We washed the two new nanoparticles batches.
- New nanoparticles DLS measurements
- Microscopy (visualisation of the new loaded NPs)
- Anti-strepta resistance check