Team:EPF Lausanne/Calendar/14 August 2013

From 2013.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
<font size = "4"> Cell Surface Display </font> <BR>
 +
 +
''BBa_K523013 Characterisation'' <br>
 +
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.
 +
<br><br>
<font size = "4"> Sensing-Effector </font> <BR>
<font size = "4"> Sensing-Effector </font> <BR>
Line 7: Line 12:
''Streaking Stabculture'' <BR>
''Streaking Stabculture'' <BR>
-
-Along with the plasmids we recieved a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.
+
-Along with the plasmids we received a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.
<BR> <BR>
<BR> <BR>

Latest revision as of 21:49, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

BBa_K523013 Characterisation
Characterisation of the iGEM plasmid BBa_K523013 we decided to use.

Sensing-Effector

Amplifying Plasmids containing gelatinases
-We transformed two cultures each with a plasmid containing a gene that encoded either MMP2 or MMP9 (two gelatinases).

Streaking Stabculture
-Along with the plasmids we received a stabculture already transformed with the two enzyme-plasmids. We streaked these cultures on LB+Ampicillin plates and let them grow over night.

Nanoparticles

Making Nanoparticles, 2nd try
-We used a new protocol.