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<br>Gibson Assembly from the DpnI digested products (see under protocols). | <br>Gibson Assembly from the DpnI digested products (see under protocols). | ||
<br> Gel electrophoresis to verify the Gibson product. | <br> Gel electrophoresis to verify the Gibson product. | ||
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| + | '''Nanoparticles''' | ||
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| + | Make nanoparticles: try 1 (second cross-linking step with glutaraldehyde of the GPs+dye). | ||
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{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Gibson Assembly:
We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids.
Gibson Assembly from the DpnI digested products (see under protocols).
Gel electrophoresis to verify the Gibson product.
Nanoparticles
Make nanoparticles: try 1 (second cross-linking step with glutaraldehyde of the GPs+dye).
"
