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'''Cell Surface Display''' | '''Cell Surface Display''' | ||
| - | ''Gibson Assembly | + | ''Gibson Assembly '' |
| - | <br> We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids. | + | <br> -We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids. |
| - | <br>Gibson Assembly from the DpnI digested products (see under protocols). | + | <br>-Gibson Assembly from the DpnI digested products (see under protocols). |
| - | <br> Gel electrophoresis to verify the Gibson product. | + | <br> -Gel electrophoresis to verify the Gibson product. |
| - | '''Nanoparticles''' | + | '''Nanoparticles''' <BR> |
| - | + | ''Making nanoparticles 1st try'' <br> | |
| + | We did the second cross-linking step with glutaraldehyde of the GPs+dye. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Gibson Assembly
-We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids.
-Gibson Assembly from the DpnI digested products (see under protocols).
-Gel electrophoresis to verify the Gibson product.
Nanoparticles
Making nanoparticles 1st try
We did the second cross-linking step with glutaraldehyde of the GPs+dye.
"
