Team:EPF Lausanne/Calendar/1 August 2013

From 2013.igem.org

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<br>-Gibson Assembly from the DpnI digested products (see under protocols).
<br>-Gibson Assembly from the DpnI digested products (see under protocols).
<br> -Gel electrophoresis to verify the Gibson product.
<br> -Gel electrophoresis to verify the Gibson product.
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'''Nanoparticles''' <BR>
'''Nanoparticles''' <BR>

Revision as of 14:37, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Assembly
-We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids.
-Gibson Assembly from the DpnI digested products (see under protocols).
-Gel electrophoresis to verify the Gibson product.


Nanoparticles

Making nanoparticles 1st try
We did the second cross-linking step with glutaraldehyde of the GPs+dye.