GigiGoesGugu (Talk | contribs) |
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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
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''Gibson Assembly '' | ''Gibson Assembly '' | ||
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<br>-Gibson Assembly from the DpnI digested products (see under protocols). | <br>-Gibson Assembly from the DpnI digested products (see under protocols). | ||
<br> -Gel electrophoresis to verify the Gibson product. | <br> -Gel electrophoresis to verify the Gibson product. | ||
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''Making nanoparticles 1st try'' <br> | ''Making nanoparticles 1st try'' <br> | ||
Gibson Assembly
-We did the DpnI digestion of our previous PCR products (streptavidin dead and INP_construct) to remove any carry over of the original plasmids.
-Gibson Assembly from the DpnI digested products (see under protocols).
-Gel electrophoresis to verify the Gibson product.
Nanoparticles
Making nanoparticles 1st try
We did the second cross-linking step with glutaraldehyde of the GPs+dye.
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