Team:EPF Lausanne/Calendar/1 September 2013

From 2013.igem.org

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Sensing <BR>
 
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'''Suspending Primers''' <BR>
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<font size = "4"> Sensing </font> <BR>
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Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.
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'''Inducing and measuring GFP expression''' <BR>
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''Suspending Primers'' <BR>
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The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.
+
-Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.
 +
<BR>
 +
''Inducing and measuring GFP expression'' <BR>
 +
-The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.
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<BR>
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'''PCR of Backbones''' <BR>
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''PCR of Backbones'' <BR>
We tried to amplify the backbones of the sensning and effector constructs by using the new primers. It did not work.
We tried to amplify the backbones of the sensning and effector constructs by using the new primers. It did not work.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 16:29, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header


Sensing

Suspending Primers
-Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.

Inducing and measuring GFP expression
-The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.


PCR of Backbones
We tried to amplify the backbones of the sensning and effector constructs by using the new primers. It did not work.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/1_September_2013"