Team:EPF Lausanne/Calendar/1 September 2013


Revision as of 21:02, 3 October 2013 by Nadia7788 (Talk | contribs)

Taxi.Coli: Smart Drug Delivery iGEM EPFL



Suspending Primers
-Our new primers arrived and we resuspended them and made primer mixes for each pair, one foreward and one reverse.

Inducing and measuring GFP expression
-The AraC-GFP containing bacteria were made to express GFP by adding 1% of 20% L-Arabinose. After adding the Arabinose we incubated them for 2.5h at 37°C and then looked at them in the microscope. They clearly fluoresce, indicating that GFP is expressed induced by addition of Arabinose to the medium.

PCR of Backbones
-We tried to amplify the backbones of the sensning and effector constructs by using the new primers. It did not work.