Team:EPF Lausanne/Calendar/20 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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''Gibson Assembly'' <br>
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We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).
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<font size = "4"> Sensing-Effector </font> <BR>
<font size = "4"> Sensing-Effector </font> <BR>

Revision as of 09:13, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Assembly
We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).

Sensing-Effector

Gradient PCR of Backbones
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.

Nanoparicles

Making Nanoparticles, 2nd try
- Biotinylation of the new nanoparticles.
- Dialysis of biotinylated nanoparticles.