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Gibson Assembly

- We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).
- Verification of the gibson reaction with a 0.8% gel electrophoresis.

Sensing-Effector

Gradient PCR of Backbones
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.

Nanoparicles

Making Nanoparticles, 2nd try
- Biotinylation of the new nanoparticles.
- Dialysis of biotinylated nanoparticles.