Team:EPF Lausanne/Calendar/20 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
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Sensing <BR>
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''Gibson Assembly'' <br>
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- We did the streptavidin alive gibson assembly reaction (NEB protocol).
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<br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.
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<br>- Gibson Assembly probably didn't work.
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<br><br>
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'''Gradient PCR of Backbones''' <BR>
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<font size = "4"> Sensing-Effector </font> <BR>
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To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.
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 +
''Gradient PCR of Backbones'' <BR>
 +
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the right size.
 +
<BR> <BR>
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'''Nanoparticles'''
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<font size = "4"> Nanoparicles</font> <BR>
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''Making Nanoparticles, 2nd try''
<BR>- Biotinylation of the new nanoparticles.
<BR>- Biotinylation of the new nanoparticles.
<BR>- Dialysis of biotinylated nanoparticles.  
<BR>- Dialysis of biotinylated nanoparticles.  
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Latest revision as of 21:56, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Gibson Assembly
- We did the streptavidin alive gibson assembly reaction (NEB protocol).
- Verification of the gibson reaction with a 0.8% gel electrophoresis.
- Gibson Assembly probably didn't work.

Sensing-Effector

Gradient PCR of Backbones
-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the right size.

Nanoparicles

Making Nanoparticles, 2nd try
- Biotinylation of the new nanoparticles.
- Dialysis of biotinylated nanoparticles.