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Team:EPF Lausanne/Calendar/20 August 2013 - Revision history
2024-03-29T04:55:11Z
Revision history for this page on the wiki
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Leabernier at 21:56, 4 October 2013
2013-10-04T21:56:05Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gradient PCR of Backbones'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gradient PCR of Backbones'' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the <del class="diffchange diffchange-inline">rigth </del>size.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the <ins class="diffchange diffchange-inline">right </ins>size.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR> <BR></div></td></tr>
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Leabernier
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=289181&oldid=prev
Sandelisa90 at 09:59, 4 October 2013
2013-10-04T09:59:01Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- We did the streptavidin alive gibson assembly reaction (<del class="diffchange diffchange-inline">50µl, 68°C, without DMSO</del>).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- We did the streptavidin alive gibson assembly reaction (<ins class="diffchange diffchange-inline">NEB protocol</ins>).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Gibson Assembly probably didn't work.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Gibson Assembly probably didn't work.</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=289006&oldid=prev
Sandelisa90 at 09:44, 4 October 2013
2013-10-04T09:44:40Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- We did the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- We did the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- Gibson Assembly probably didn't work.</ins></div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=288787&oldid=prev
Sandelisa90 at 09:23, 4 October 2013
2013-10-04T09:23:17Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- We <del class="diffchange diffchange-inline">repeated </del>the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- We <ins class="diffchange diffchange-inline">did </ins>the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=288729&oldid=prev
Sandelisa90 at 09:16, 4 October 2013
2013-10-04T09:16:45Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br></del>- We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis.</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=288707&oldid=prev
Sandelisa90 at 09:14, 4 October 2013
2013-10-04T09:14:59Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gibson Assembly'' <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>- </ins>We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO)<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br>- Verification of the gibson reaction with a 0.8% gel electrophoresis</ins>.</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=288693&oldid=prev
Sandelisa90 at 09:13, 4 October 2013
2013-10-04T09:13:46Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Cell Surface Display </font> <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Gibson Assembly'' <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We repeated the streptavidin alive gibson assembly reaction (50µl, 68°C, without DMSO).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=281366&oldid=prev
GigiGoesGugu at 19:06, 3 October 2013
2013-10-03T19:06:12Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><font size = "4"> Sensing </font> <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><font size = "4"> Sensing<ins class="diffchange diffchange-inline">-Effector </ins></font> <BR></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gradient PCR of Backbones'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Gradient PCR of Backbones'' <BR></div></td></tr>
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GigiGoesGugu
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=278152&oldid=prev
GigiGoesGugu at 15:53, 3 October 2013
2013-10-03T15:53:28Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Sensing </font> <BR></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Sensing </del><BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''Gradient PCR of Backbones'' <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><BR> </ins><BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''Gradient PCR of Backbones''' </del><BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><font size = "4"> Nanoparicles</font> </ins><BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">To check which annealing temperature is ideal for our backbone amplification we did a gradient PCR. None of the temperatures seemed to work. Furthermore, the band that we get is still not at the rigth size.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''<ins class="diffchange diffchange-inline">Making </ins>Nanoparticles<ins class="diffchange diffchange-inline">, 2nd try</ins>''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'</del>''Nanoparticles<del class="diffchange diffchange-inline">'</del>''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- Biotinylation of the new nanoparticles.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- Biotinylation of the new nanoparticles.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- Dialysis of biotinylated nanoparticles. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- Dialysis of biotinylated nanoparticles. </div></td></tr>
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GigiGoesGugu
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/20_August_2013&diff=270812&oldid=prev
Leabernier at 21:01, 2 October 2013
2013-10-02T21:01:13Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:01, 2 October 2013</td>
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Leabernier