Team:EPF Lausanne/Calendar/21 August 2013

From 2013.igem.org

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<font size = "4"> Sensing </font> <BR>
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<font size = "4"> Cell Surface Display </font> <BR>
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''Transformation'' <br>
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Transformation of DH5alpha competent cells with the streptavidin alive gibson potentially non working product.
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<br><br>
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<font size = "4"> Sensing-Effector </font> <BR>
''Transformation of Bacteria'' <BR>
''Transformation of Bacteria'' <BR>
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We did another transformation: <BR>
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-We did another transformation: <BR>
1.) Two plates with the AraC promoter containing Plasmid, directly taken from the Kit stock <BR>
1.) Two plates with the AraC promoter containing Plasmid, directly taken from the Kit stock <BR>
2.) Two plates with the pH promoter, directly taken from the Kit stock <BR>
2.) Two plates with the pH promoter, directly taken from the Kit stock <BR>
3.) Two plates with another plasmid with only the pBAd promoter <BR>
3.) Two plates with another plasmid with only the pBAd promoter <BR>
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4.) Two plastes as a transformation efficiency control with the puc19 plasmid <BR>
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4.) Two plates as a transformation efficiency control with the puc19 plasmid <BR>
5.) Two plates as a negative control with untransformed bacteria on chloramphenicol plates <BR>
5.) Two plates as a negative control with untransformed bacteria on chloramphenicol plates <BR>
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''Making Nanoparticles, 2nd try''<BR>
''Making Nanoparticles, 2nd try''<BR>
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We finished the dialysis of the biotinylated nanoparticles. Looks good; the solution is white.  
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-We finished the dialysis of the biotinylated nanoparticles. Looks good; the solution is white.  
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Latest revision as of 21:56, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Transformation
Transformation of DH5alpha competent cells with the streptavidin alive gibson potentially non working product.

Sensing-Effector

Transformation of Bacteria
-We did another transformation:
1.) Two plates with the AraC promoter containing Plasmid, directly taken from the Kit stock
2.) Two plates with the pH promoter, directly taken from the Kit stock
3.) Two plates with another plasmid with only the pBAd promoter
4.) Two plates as a transformation efficiency control with the puc19 plasmid
5.) Two plates as a negative control with untransformed bacteria on chloramphenicol plates


Nanoparticles

Making Nanoparticles, 2nd try
-We finished the dialysis of the biotinylated nanoparticles. Looks good; the solution is white.