GigiGoesGugu (Talk | contribs) |
Leabernier (Talk | contribs) |
||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| - | + | <font size = "4">Cell Surface Display</font> | |
''PCR optimisation'' <BR> | ''PCR optimisation'' <BR> | ||
| - | To improve the ''pINP_construct'' amplification we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. | + | To improve the ''pINP_construct'' amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis. |
| - | + | <BR><BR> | |
| - | + | ||
| - | + | <font size = "4">General</font> | |
| - | + | <BR>Competent cells try 3: it worked! | |
| - | Competent cells try 3: it worked! | + | |
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
PCR optimisation
To improve the pINP_construct amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis.
General
Competent cells try 3: it worked!
"
