Team:EPF Lausanne/Calendar/25 July 2013


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Taxi.Coli: Smart Drug Delivery iGEM EPFL


Cell Surface Display

PCR optimisation
To improve the pINP_construct amplification, we did a gradient PCR at 72°C, 73°C and 74°C with 3% and 5% DMSO added to the mix. We verified our results with a 0.8% gel electrophoresis.

Competent cells try 3: it worked!