Team:EPF Lausanne/Calendar/25 October 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display</font>
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<BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR>
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1) Third PCRs (50µl)
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<br>- For IYS: PCR (5) to add Gibson overhangs on BBa_K523013_PCR_product (3) where linker were added the day before
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<br>- For ISY: PCR (6) to add Gibson overhangs on BBa_K523013_PCR_product (4) where linker were added the day before
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<br>- PCR purification
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2) Gibson Assembly
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<br>- IYS Gibson Assembly using PCR (1) from the day before and PCR (5)
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<br>- ISY Gibson Assembly using PCR (2) from the day before and PCR (6)
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<b>
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<br>- 0.8% Gel electrophoresis to verify the thirs PCRs and the Gibson Products
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<br><br>
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Revision as of 15:45, 27 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

1) Third PCRs (50µl)
- For IYS: PCR (5) to add Gibson overhangs on BBa_K523013_PCR_product (3) where linker were added the day before
- For ISY: PCR (6) to add Gibson overhangs on BBa_K523013_PCR_product (4) where linker were added the day before
- PCR purification

2) Gibson Assembly
- IYS Gibson Assembly using PCR (1) from the day before and PCR (5)
- ISY Gibson Assembly using PCR (2) from the day before and PCR (6)


- 0.8% Gel electrophoresis to verify the thirs PCRs and the Gibson Products