Team:EPF Lausanne/Calendar/25 October 2013

From 2013.igem.org

(Difference between revisions)
Line 7: Line 7:
<br>- For ISY: PCR (6) to add Gibson overhangs on BBa_K523013_PCR_product (4) where linker were added the day before
<br>- For ISY: PCR (6) to add Gibson overhangs on BBa_K523013_PCR_product (4) where linker were added the day before
<br>- PCR purification
<br>- PCR purification
-
 
+
<br><br>
2) Gibson Assembly
2) Gibson Assembly
<br>- IYS Gibson Assembly using PCR (1) from the day before and PCR (5)
<br>- IYS Gibson Assembly using PCR (1) from the day before and PCR (5)

Revision as of 15:47, 27 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

1) Third PCRs (50µl)
- For IYS: PCR (5) to add Gibson overhangs on BBa_K523013_PCR_product (3) where linker were added the day before
- For ISY: PCR (6) to add Gibson overhangs on BBa_K523013_PCR_product (4) where linker were added the day before
- PCR purification

2) Gibson Assembly
- IYS Gibson Assembly using PCR (1) from the day before and PCR (5)
- ISY Gibson Assembly using PCR (2) from the day before and PCR (6)


- 0.8% Gel electrophoresis to verify the thirs PCRs and the Gibson Products