Team:EPF Lausanne/Calendar/26 August 2013

From 2013.igem.org

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<font size = "4"> Sensing </font> <BR>
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Sensing <BR>
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''PCR of the BBa_J23119 part from iGEM'' <BR>
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-In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid. <BR>
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'''PCR of the BBa_J23119 part from iGEM''' <BR>
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In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid. <BR>
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The PCR of this promoter worked very well.
The PCR of this promoter worked very well.

Revision as of 16:02, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

PCR of the BBa_J23119 part from iGEM
-In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid.
The PCR of this promoter worked very well.