Team:EPF Lausanne/Calendar/26 August 2013

From 2013.igem.org

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<font size = "4"> Cell Surface Display </font> <BR>
<font size = "4"> Cell Surface Display </font> <BR>
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All the samples were sent for sequencing from their miniprep.
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Working samples were sent for sequencing from their miniprep with VR primers.
''Begining of functionnal assays'' <br>
''Begining of functionnal assays'' <br>
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INP_Streptavidin Alive, Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.
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INP_Streptavidin Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.
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Revision as of 09:50, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Working samples were sent for sequencing from their miniprep with VR primers.

Begining of functionnal assays
INP_Streptavidin Dead and iGEM overnight inoculation (LB + chloramphenicol) and DH5alpha competent cells from glycerol stocks plates.

Sensing-Effector

PCR of the BBa_J23119 part from iGEM
-In order to have a positive control for our sensing constructs we wanted to use a constitutive promoter that would express the superfolded GFP all the time. For this we used a promoter from iGEM which we inserted instead of the arabinose sensitive promoter into the pSB1C3 plasmid.
The PCR of this promoter worked very well.