http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/26_October_2013&feed=atom&action=history
Team:EPF Lausanne/Calendar/26 October 2013 - Revision history
2024-03-28T12:19:46Z
Revision history for this page on the wiki
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http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/26_October_2013&diff=353793&oldid=prev
Sandelisa90 at 23:06, 28 October 2013
2013-10-28T23:06:54Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Colonies inoculation for microscopy</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Colonies inoculation for microscopy</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>- Inoculation in 3ml LB + Chloramphenicol of the 16 gibson assembly products and the 2 BBa_K523013 miniprep colonies.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>- Inoculation in 3ml LB + Chloramphenicol <ins class="diffchange diffchange-inline">+ IPTG </ins>of the 16 gibson assembly products and the 2 BBa_K523013 miniprep colonies.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Inoculation in 3ml LB of normal competent cells to be used as negative controls</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- Inoculation in 3ml LB of normal competent cells to be used as negative controls</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/26_October_2013&diff=338497&oldid=prev
Sandelisa90 at 17:09, 27 October 2013
2013-10-27T17:09:06Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Cell Surface Display</font> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The transformation from the day before worked. There were no colonies is the insert and the competent cells control as expected.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">There were colonies in the backbones control and in the gibson transformation plates.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">1) Colony PCR </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br> we used one Fw primer we designed, annealing inside the INP sequence and the RV primer from iGEM registry. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br> Tm = 61°C using Phusion polymerase and 1min30sec extension time.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- 8 colonies from G.A. IYS plate</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- 8 colonies from G.A. ISY plate</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- 2 colonies respectively from IYS and ISY backbone controls plates</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- 2 colonies from the BBa_K523013 miniprep template transformation plate </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2) Colonies inoculation for microscopy</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- Inoculation in 3ml LB + Chloramphenicol of the 16 gibson assembly products and the 2 BBa_K523013 miniprep colonies.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- Inoculation in 3ml LB of normal competent cells to be used as negative controls</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td></tr>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/26_October_2013&diff=338411&oldid=prev
Sandelisa90 at 16:58, 27 October 2013
2013-10-27T16:58:10Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:58, 27 October 2013</td>
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Sandelisa90
http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/26_October_2013&diff=256922&oldid=prev
Sophierivara: Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}"
2013-09-30T22:27:31Z
<p>Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}"</p>
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