http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&feed=atom&action=historyTeam:EPF Lausanne/Calendar/28 August 2013 - Revision history2024-03-28T13:21:50ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=306087&oldid=prevMareikeapelt at 22:33, 4 October 20132013-10-04T22:33:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Functional/Characterisation Assays'' <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Functional/Characterisation Assays'' <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- 1ml of inoculated samples (ISA, ISD, ISI and CC) were pelleted down, resuspended in PBS and incubated 1hr with anti-streptavidin FITC conjugated antibody.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>- 1ml of inoculated samples (ISA, ISD, ISI and CC) were pelleted down, resuspended in PBS and incubated 1hr with anti-streptavidin FITC conjugated antibody.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br>- The cells were then fix or kept in liquid <del class="diffchange diffchange-inline">(see protocols) </del>medium and visualized under the microscope.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br>- The cells were then fix or kept in liquid medium and visualized under the microscope.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- No fluorescence was observed. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- No fluorescence was observed. </div></td></tr>
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</table>Mareikeapelthttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=305192&oldid=prevLeabernier at 22:03, 4 October 20132013-10-04T22:03:08Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Cell Surface Display </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Cell Surface Display </font> <BR></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''<del class="diffchange diffchange-inline">Functionnal</del>/Characterisation Assays'' <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''<ins class="diffchange diffchange-inline">Functional</ins>/Characterisation Assays'' <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>- 1ml of inoculated samples (ISA, ISD, ISI and CC) were pelleted down, <del class="diffchange diffchange-inline">resupended </del>in PBS and incubated 1hr with anti-streptavidin FITC conjugated antibody.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- 1ml of inoculated samples (ISA, ISD, ISI and CC) were pelleted down, <ins class="diffchange diffchange-inline">resuspended </ins>in PBS and incubated 1hr with anti-streptavidin FITC conjugated antibody.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- The cells were then fix or kept in liquid (see protocols) medium and visualized under the microscope.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- The cells were then fix or kept in liquid (see protocols) medium and visualized under the microscope.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- No fluorescence was observed. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br>- No fluorescence was observed. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>-We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we <del class="diffchange diffchange-inline">innoculated </del>two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>-We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we <ins class="diffchange diffchange-inline">inoculated </ins>two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=288957&oldid=prevSandelisa90 at 09:40, 4 October 20132013-10-04T09:40:11Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Cell Surface Display </font> <BR></ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Functionnal/Characterisation Assays'' <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">- 1ml of inoculated samples (ISA, ISD, ISI and CC) were pelleted down, resupended in PBS and incubated 1hr with anti-streptavidin FITC conjugated antibody.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- The cells were then fix or kept in liquid (see protocols) medium and visualized under the microscope.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br>- No fluorescence was observed. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font size = "4"> Sensing-Effector </font> <BR></div></td></tr>
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</table>Sandelisa90http://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=281415&oldid=prevGigiGoesGugu at 19:08, 3 October 20132013-10-03T19:08:02Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><font size = "4"> Sensing </font> <BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><font size = "4"> Sensing<ins class="diffchange diffchange-inline">-Effector </ins></font> <BR></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=278369&oldid=prevGigiGoesGugu at 16:06, 3 October 20132013-10-03T16:06:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Checking GFP expression induced by arabinose'' <BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we innoculated two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>-We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we innoculated two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Resuspending K-12, MG1655 e.coli'' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Resuspending K-12, MG1655 e.coli'' <BR></div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=278362&oldid=prevGigiGoesGugu at 16:06, 3 October 20132013-10-03T16:06:30Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><font size = "4"> Sensing </font> <BR></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Sensing </del><BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">''Checking GFP expression induced by arabinose'' </ins><BR></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">-We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we innoculated two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">'''Checking GFP expression induced by arabinose''' <BR></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we innoculated two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Resuspending K-12, MG1655 e.coli'' <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">-Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><BR> <BR></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'''Resuspending K-12, MG1655 e.coli''' </del><BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><font size = "4"> Nanoparticles</font> </ins><BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''<del class="diffchange diffchange-inline">'</del>Nanoparticles<del class="diffchange diffchange-inline">'</del>''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''<ins class="diffchange diffchange-inline">Digestion of the </ins>Nanoparticles''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><BR></del>- First try for the digestion with MMP2 : 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>- First try for the digestion with <ins class="diffchange diffchange-inline">matrix metalloprotease </ins>MMP2: 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media. <ins class="diffchange diffchange-inline">We did two </ins>measurements <ins class="diffchange diffchange-inline">of the size of the nanoparticles</ins>: the nanoparticles <ins class="diffchange diffchange-inline">became </ins>a little bit smaller in the presence of MMP2<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><BR>- 2 </del>measurements <del class="diffchange diffchange-inline">done </del>: the nanoparticles <del class="diffchange diffchange-inline">become </del>a little bit smaller in the presence of MMP2 </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=270909&oldid=prevLeabernier at 21:10, 2 October 20132013-10-02T21:10:25Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Nanoparticles'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Nanoparticles'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- First try for the digestion with MMP2 : 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- First try for the digestion with MMP2 : 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- 2 measurements done : the nanoparticles become a little bit smaller in the presence of MMP2 </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><BR>- 2 measurements done : the nanoparticles become a little bit smaller in the presence of MMP2 </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=270903&oldid=prevLeabernier at 21:10, 2 October 20132013-10-02T21:10:08Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:10, 2 October 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Resuspending K-12, MG1655 e.coli''' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Resuspending K-12, MG1655 e.coli''' <BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Nanoparticles'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><BR>- First try for the digestion with MMP2 : 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><BR>- 2 measurements done : the nanoparticles become a little bit smaller in the presence of MMP2 </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td></tr>
</table>Leabernierhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=255362&oldid=prevGigiGoesGugu at 18:22, 30 September 20132013-09-30T18:22:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Resuspending K-12, MG1655 e.coli''' <BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Resuspending K-12, MG1655 e.coli''' <BR></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module<ins class="diffchange diffchange-inline">. We resuspended this strain according to the manufacturer's instructions and let them grow over night</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td></tr>
</table>GigiGoesGuguhttp://2013.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Calendar/28_August_2013&diff=255359&oldid=prevGigiGoesGugu at 18:21, 30 September 20132013-09-30T18:21:18Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Resuspending K-12, MG1655 e.coli''' <BR></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:EPFL2013Footer}}</div></td></tr>
</table>GigiGoesGugu