Team:EPF Lausanne/Calendar/28 August 2013

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Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Checking GFP expression induced by arabinose
We wanted to induce simple GFP expression in bacteria transformed with the AraC-GFP plasmid to check if the bacteria really did contain the plasmid and also if it worked the way it is supposed to. In order to do so, we innoculated two cultures into a chloramphenicol-Arabinose containing medium and let them grow over night.


Resuspending K-12, MG1655 e.coli
Today we also recieved the E.Coli strain which contained two of the three promoter that we wanted to use for our sensing module. We resuspended this strain according to the manufacturer's instructions and let them grow over night.

Nanoparticles
- First try for the digestion with MMP2 : 2 different media were used, Tris/HCl with NaCl and CaCl2 and cell media.
- 2 measurements done : the nanoparticles become a little bit smaller in the presence of MMP2