Team:EPF Lausanne/Calendar/28 October 2013

From 2013.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
<font size = "4"> Cell Surface Display</font>
 +
<BR>''CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)'' <BR>
 +
 +
Repeating the microscopy
 +
<br>- samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
 +
<br>- different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
 +
<br>- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.
 +
 +
<br><br>
 +
<font size = "4"> Nanoparticles</font> <BR>
<font size = "4"> Nanoparticles</font> <BR>

Revision as of 23:13, 28 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
CLoning INP_EYFP_Streptavidin (IYS) and INP_Streptavidin_EYFP (ISY)

Repeating the microscopy
- samples 1 for IYS and 3 for ISY were chosed and prepared as the day before based on our protocols
- different aliquots of these samples were incubated separatly with a FITC conjugated anti-streptavidin antibody; a Phicoerythrin conjugated anti-GFP antibody; biotynilated nanoparticles and at the end with biotin conjugated anti-GFP antibody (revealed with avidin dylight (red)).
- Some images of this microscopy are on our wiki page. It shows that the construct is expressed and that YFP is likely to be on the outer membrane.



Nanoparticles

Microfluidic
- Repeat microfluidics experiment for nanoparticles digestion with MMP2

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/28_October_2013"