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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | <font size = "4"> Cell Surface Display </font> <BR> | ||
| - | '' | + | ''Streptavidin Alive Gibson''<br> |
| + | - Since the transformation with the previous gibson product didn't work, we decided to repeat the streptavidin Alive PCR to isolate the coding sequence. | ||
| + | <br>- 50µl, 68°C, without DMSO. | ||
| + | <br>- 0.8% electrophoresis gel to verify the reaction product. We had the right product. | ||
| + | <br>- PCR purification. | ||
| + | <br><br> | ||
| - | More measurements made for the MMP2 digestion | + | <font size = "4"> Nanoparticles</font> <BR> |
| + | |||
| + | ''Digestion of Nanoparticles''<BR> | ||
| + | More measurements were made for the MMP2 digestion but the nanoparticles did not get much smaller. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Streptavidin Alive Gibson
- Since the transformation with the previous gibson product didn't work, we decided to repeat the streptavidin Alive PCR to isolate the coding sequence.
- 50µl, 68°C, without DMSO.
- 0.8% electrophoresis gel to verify the reaction product. We had the right product.
- PCR purification.
Nanoparticles
Digestion of Nanoparticles
More measurements were made for the MMP2 digestion but the nanoparticles did not get much smaller.
"
