Sandelisa90 (Talk | contribs) |
Sandelisa90 (Talk | contribs) |
||
| Line 3: | Line 3: | ||
''Streptavidin Alive Gibson''<br> | ''Streptavidin Alive Gibson''<br> | ||
| - | Since the transformation with the previous gibson product didn't work, we decided to repeat the streptavidin Alive PCR to isolate the | + | - Since the transformation with the previous gibson product didn't work, we decided to repeat the streptavidin Alive PCR to isolate the coding sequence. |
| - | + | <br>- 50µl, 68°C, without DMSO. | |
| - | + | <br>- 0.8% electrophoresis gel to verify the reaction product. We had the right product. | |
| + | <br>- PCR purification. | ||
<br><br> | <br><br> | ||
Streptavidin Alive Gibson
- Since the transformation with the previous gibson product didn't work, we decided to repeat the streptavidin Alive PCR to isolate the coding sequence.
- 50µl, 68°C, without DMSO.
- 0.8% electrophoresis gel to verify the reaction product. We had the right product.
- PCR purification.
Nanoparticles
Digestion of Nanoparticles
More measurements were made for the MMP2 digestion but the nanoparticles did not get much smaller.
"
