Team:EPF Lausanne/Calendar/30 August 2013

From 2013.igem.org

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Sensing <BR>
 
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'''Isolation of genomic MG1655 DNA''' <BR>
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<font size = "4"> Sensing </font> <BR>
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We followed the manufactures instruction from the DNa isolation kit we recieved in order to isolate the genomic DNA. The yields were within the expected Range, from 120ng/ul-300ng/ul.
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'''Nanoparticles'''
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''Isolation of genomic MG1655 DNA'' <BR>
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<BR>- 1 more measurement : still the same
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-We followed the manufactures instruction from the DNa isolation kit we recieved in order to isolate the genomic DNA. The yields were within the expected Range, from 120ng/ul-300ng/ul.
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<BR>- Second try for the digestion with MMP2 : only with the Tris buffer, but with a higher concentration of MMP2
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<BR>- 3 measurements done for the second try : the nanoparticles get smaller with MMP2, more clearly than with the first try  
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<font size = "4"> Sensing </font> <BR>
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''Digestion of nanoparticles'' <BR>
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We did one more measurement of the solution containing our nanoparticles and MMP2. The result showed that the Nanoparticles were still the same size.
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<BR>- We did a second try for the digestion with MMP2: only with the Tris buffer, but with a higher concentration of MMP2
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<BR>- Three measurements were done for the second try: the nanoparticles got smaller with MMP2, more clearly than during the first try  
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Revision as of 16:12, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header


Sensing

Isolation of genomic MG1655 DNA
-We followed the manufactures instruction from the DNa isolation kit we recieved in order to isolate the genomic DNA. The yields were within the expected Range, from 120ng/ul-300ng/ul.


Sensing

Digestion of nanoparticles
We did one more measurement of the solution containing our nanoparticles and MMP2. The result showed that the Nanoparticles were still the same size.
- We did a second try for the digestion with MMP2: only with the Tris buffer, but with a higher concentration of MMP2
- Three measurements were done for the second try: the nanoparticles got smaller with MMP2, more clearly than during the first try

Wiki Links

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