Team:EPF Lausanne/Calendar/30 July 2013

From 2013.igem.org

(Difference between revisions)
Line 5: Line 5:
<br> We did the ''final PCR'' of ''pINP_construct'' with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
<br> We did the ''final PCR'' of ''pINP_construct'' with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
<br> We did again a 0.8% electrophoresis gel to verify the PCR product.  
<br> We did again a 0.8% electrophoresis gel to verify the PCR product.  
 +
<br> Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 15:09, 1 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin: inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, 8hrs to make a glycerol Stock.
We did the final PCR of pINP_construct with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
We did again a 0.8% electrophoresis gel to verify the PCR product.
Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.