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{{Template:EPFL2013Header}} | {{Template:EPFL2013Header}} | ||
| + | <font size = "4">Cell Surface Display</font> | ||
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| + | ''Streptavidin'' <BR> | ||
| + | -Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock. <BR> | ||
| + | -Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after. | ||
| + | <br> -We did the ''final PCR'' of ''pINP_construct'' with the following optimal condititons: volume 50µl, 76°C, 5% DMSO. | ||
| + | <br> -We did a 0.8% electrophoresis gel to verify the PCR product. | ||
{{Template:EPFL2013Footer}} | {{Template:EPFL2013Footer}} | ||
Streptavidin
-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock.
-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.
-We did the final PCR of pINP_construct with the following optimal condititons: volume 50µl, 76°C, 5% DMSO.
-We did a 0.8% electrophoresis gel to verify the PCR product.
"
