Team:EPF Lausanne/Calendar/30 July 2013

From 2013.igem.org

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'''Cell Surface Display'''
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''Streptavidin'' <BR>
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-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock. <BR>
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-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.
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<br> -We did the ''final PCR'' of ''pINP_construct'' with the following optimal condititons: volume 50µl, 76°C, 5% DMSO.
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<br> -We did a 0.8% electrophoresis gel to verify the PCR product.
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''Streptavidin:'' inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, 8hrs to make a glycerol Stock.
 
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<br> We did the ''final PCR'' of ''pINP_construct'' with the following optimum condiitons: volume 50µl, 76°C, 5% DMSO.
 
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<br> we did again a 0.8% electrophoresis gel to verify the PCR product.
 
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Latest revision as of 14:41, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin
-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, we incubated them for 8h to make a glycerol Stock.
-Inoculation of the pET21a Streptavidin Dead we received in 3ml LB + Amp, overnight (16hrs) to make a miniprep the day after.
-We did the final PCR of pINP_construct with the following optimal condititons: volume 50µl, 76°C, 5% DMSO.
-We did a 0.8% electrophoresis gel to verify the PCR product.