Team:EPF Lausanne/Calendar/31 July 2013

From 2013.igem.org

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'''Cell Surface Display'''
'''Cell Surface Display'''
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''Streptavidin Dead Cloning: '' Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
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''Streptavidin Dead Cloning '' <BR>
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<br> PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
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-Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
-
<br> We did a 0.8% electrophoresis gel to verify the reaction.
+
<br> -PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
-
<br> As the gel result was good we did a larger amount (50µl) PCR at 68°C.
+
<br> -We did a 0.8% electrophoresis gel to verify the reaction.
-
<br> We did another Gel to check the product.
+
<br> -As the gel result was good we did a larger amount (50µl) PCR at 68°C.
-
<br> We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.
+
<br> -We did another Gel to check the product.
 +
<br> -We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.
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{{Template:EPFL2013Footer}}

Revision as of 14:34, 3 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display

Streptavidin Dead Cloning
-Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
-PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
-We did a 0.8% electrophoresis gel to verify the reaction.
-As the gel result was good we did a larger amount (50µl) PCR at 68°C.
-We did another Gel to check the product.
-We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.