Team:EPF Lausanne/Calendar/31 July 2013

From 2013.igem.org

(Difference between revisions)
(Created page with "{{Template:EPFL2013Header}} {{Template:EPFL2013Footer}}")
Line 1: Line 1:
{{Template:EPFL2013Header}}
{{Template:EPFL2013Header}}
 +
'''Cell Surface Display'''
 +
''Streptavidin Dead Cloning: '' Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
 +
<br> PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
 +
<br> We did a 0.8% electrophoresis gel to verify the reaction.
 +
<br> As the gel result was good we did a larger amount (50µl) PCR at 68°C.
 +
<br> We did another Gel to check the product.
 +
<br> We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 15:07, 1 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display Streptavidin Dead Cloning: Miniprep of pET21a Streptavidin Dead cells inoculated the day before.
PCR of pET21a Streptavidn Dead to amplify the Streptavidin sequence. The reaction was at 66°C, 68°C and 70°C without DMSO.
We did a 0.8% electrophoresis gel to verify the reaction.
As the gel result was good we did a larger amount (50µl) PCR at 68°C.
We did another Gel to check the product.
We did a PCR purification (Qiagen protocol) of the streptavidin dead obtained. We measured the Concentration at the nanodrop.

Wiki Links

Retrieved from "http://2013.igem.org/Team:EPF_Lausanne/Calendar/31_July_2013"