Team:EPF Lausanne/Calendar/3 September 2013

From 2013.igem.org

(Difference between revisions)
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''Repeating Streptavidin Alive (SA) Gibson''<br>
''Repeating Streptavidin Alive (SA) Gibson''<br>
- DpnI digestion of the SA purifies PCR product to remove parental plasmid carry over.
- DpnI digestion of the SA purifies PCR product to remove parental plasmid carry over.
-
<br>- Gibson Reaction between the SA DpnI digested product (insert) and the INP_construct DpnI digested (backbone) in july.
+
<br>- Gibson Reaction between the SA DpnI digested product (insert) and the INP_construct DpnI digested (backbone) in July.
<br>- 0.8% electrophoresis gel to verify gibson product.
<br>- 0.8% electrophoresis gel to verify gibson product.
<br>- DH5alpha competent cells transformation with Gibson product with negative controls transformations (PCR backbone, PCR insert and water).
<br>- DH5alpha competent cells transformation with Gibson product with negative controls transformations (PCR backbone, PCR insert and water).
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<font size = "4"> Sensing-Effector </font> <BR>
<font size = "4"> Sensing-Effector </font> <BR>
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''Gradient PCR of sensning constructs'' <BR>
+
''Gradient PCR of sensing constructs'' <BR>
-
-We used the new primers to run a PCR on the backbones and insert of the sensning constructs. But it did not work. Because of that we decided to do a gradient PCR.
+
-We used the new primers to run a PCR on the backbones and insert of the sensing constructs. But it did not work. Because of that we decided to do a gradient PCR.
<BR><BR>
<BR><BR>
<font size = "4"> Nanoparticles</font> <BR>
<font size = "4"> Nanoparticles</font> <BR>
-
''Labelling of Nanoparticles''<BR>
+
''Labeling of Nanoparticles''<BR>
-
-We labelled the biotinylated gelatin nanoparticles with NHS-ester Cy5 dye.
+
-We labelled the biotinylated gelatin nanoparticles with NHS-ester CY5 dye.
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Revision as of 22:09, 4 October 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Cell Surface Display
Repeating Streptavidin Alive (SA) Gibson
- DpnI digestion of the SA purifies PCR product to remove parental plasmid carry over.
- Gibson Reaction between the SA DpnI digested product (insert) and the INP_construct DpnI digested (backbone) in July.
- 0.8% electrophoresis gel to verify gibson product.
- DH5alpha competent cells transformation with Gibson product with negative controls transformations (PCR backbone, PCR insert and water).

Sensing-Effector

Gradient PCR of sensing constructs
-We used the new primers to run a PCR on the backbones and insert of the sensing constructs. But it did not work. Because of that we decided to do a gradient PCR.

Nanoparticles

Labeling of Nanoparticles
-We labelled the biotinylated gelatin nanoparticles with NHS-ester CY5 dye.