Team:EPF Lausanne/Calendar/4 September 2013

From 2013.igem.org

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'''Results of the gradient PCR of the Sensing constructs from the previous day''' <BR>
'''Results of the gradient PCR of the Sensing constructs from the previous day''' <BR>
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The Gradient PCR of the Backbones for the sensning constructs did not work. So we wanted to digest the backbone with an enzyme that cuts in the region of the AraC promoter, as this part would be removed during later PCR for the sensing constructs.
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The Gradient PCR of the Backbones for the sensning constructs did not work. So we wanted to digest the backbone with an enzyme that cuts in the region of the AraC promoter, as this part would be removed during later PCR for the sensing constructs. This would yield a linear DNA which would be easier to amplify.
{{Template:EPFL2013Footer}}
{{Template:EPFL2013Footer}}

Revision as of 18:43, 30 September 2013

Taxi.Coli: Smart Drug Delivery iGEM EPFL

Header

Sensing

Results of the gradient PCR of the Sensing constructs from the previous day
The Gradient PCR of the Backbones for the sensning constructs did not work. So we wanted to digest the backbone with an enzyme that cuts in the region of the AraC promoter, as this part would be removed during later PCR for the sensing constructs. This would yield a linear DNA which would be easier to amplify.